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排序方式: 共有555条查询结果,搜索用时 140 毫秒
1.
Christina Thoisen Jakob Skov Pedersen Lars Jrgensen Anker Kuehn Benni Winding Hansen Sren Laurentius Nielsen 《Aquaculture Research》2020,51(8):3367-3375
The microalga Rhodomonas salina is widely used in aquaculture. There is a need for optimization of the growth of the microalgae and its content of essential fatty acids. Here, the fatty acid profile of Rhodomonas in relation to cell density during cultivation in a tubular PBR is investigated. It is expected that cell density is an important factor in controlling productivity and fatty acid content of the microalgae because cell density is important in determining light availability due to the self‐shading of the algae. The carbon productivity as a function of cell density is described by a saturation curve. The carbon productivity and the productivity of total fatty acids are lowest at the lowest cell density, and independent of cell density at higher cell densities. The relative contribution of the two poly‐unsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) increases with increasing cell density and saturates at 1 × 106 cells/ml. We conclude that large‐scale production of Rhodomonas in this tubular PBR should take place at cell densities of 1 × 106 cells/ml, while there are indications for increasing difficulties in maintaining steady‐state production in this PBR at higher cell densities. 相似文献
2.
Ilias S Frydas Ivan Trus Lise K Kvisgaard Caroline Bonckaert Vishwanatha RAP Reddy Yewei Li Lars E Larsen Hans J Nauwynck 《Veterinary research》2015,46(1)
In this study, the pathogenic behavior of PRRSV 13V091 and 13V117, isolated in 2013 from two different Belgian farms with enzootic respiratory problems shortly after weaning in the nursery, were compared with the Belgian strain 07V063 isolated in 2007. Full-length genome sequencing was performed to identify their origin. Twelve weeks-old pigs were inoculated intranasally (IN) with 13V091, 13V117 or 07V063 (9 pigs/group). At 10 days post inoculation (dpi), 4 animals from each group were euthanized and tissues were collected for pathology, virological and serological analysis. 13V091 infection resulted in the highest respiratory disease scores and longest period of fever. Gross lung lesions were more pronounced for 13V091 (13%), than for 13V117 (7%) and 07V063 (11%). The nasal shedding and viremia was also most extensive with 13V091. The 13V091 group showed the highest virus replication in conchae, tonsils and retropharyngeal lymph nodes. 13V117 infection resulted in the lowest virus replication in lymphoid tissues. 13V091 showed higher numbers of sialoadhesin− infected cells/mm2 in conchae, tonsils and spleen than 13V117 and 07V063. Neutralizing antibody response with 07V063 was stronger than with 13V091 and 13V117. It can be concluded that (i) 13V091 is a highly pathogenic type 1 subtype 1 PRRSV strain that replicates better than 07V063 and 13V117 and has a strong tropism for sialoadhesin− cells and (ii) despite the close genetic relationship between 13V117 and 07V063, 13V117 has an increased nasal replication and shedding, but a decreased replication in lymphoid tissues compared to 07V063.
Electronic supplementary material
The online version of this article (doi:10.1186/s13567-015-0166-3) contains supplementary material, which is available to authorized users. 相似文献3.
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- As top predators, it has been suggested that southern elephant seals serve as sentinels of ecosystem status to inform management and conservation.
- This is because southern elephant seals annually undertake two large‐scale foraging migrations for 2–3 and 7–8 months to replenish resources after fasting during breeding and moulting and often rely on dynamic macroscale latitudinal fronts to provide favourable foraging through aggregating prey.
- Yet it is largely unknown whether southern elephant seals respond to changes in frontal systems over the years, whether their foraging success is associated with specific frontal systems shifts, and how flexible southern elephant seals populations are in behaviourally adapting to changes in frontal systems.
- This study examines the relationship between frontal systems and the resource acquisition of 64 southern elephant seals during four post‐moult and three post‐breeding migrations between 2005 and 2010.
- Satellite‐relay‐data‐loggers provided in situ measurements concurrent with >27,500 dive profiles to define fronts and interfrontal zones between the Subtropical Frontal Zone and the Southern Boundary of the Antarctic Circumpolar Current. For >430,000 in situ measurements water mass properties could be identified.
- Generally, southern elephant seals associate more frequently with more southerly, higher‐latitude fronts/zones. Body condition improvements related to a given frontal system or water mass vary strongly according to year, season, month and sex.
- The variability in body condition improvements is higher in some frontal systems than in others, probably owing to shifts in the Subantarctic and Polar Front.
- During a migration, some individuals stay within ≤3 frontal systems, whilst others change between several frontal systems and primarily improve their body condition in upper ocean waters.
- Southern elephant seals do not trace particular water masses across frontal systems, and both surface and deep foraging strategies are used.
- This suggests that southern elephant seals do not target particular water masses but adjust foraging and movement strategies to exploit boundary areas at which mixing and prey aggregation is high.
- The large behavioural plasticity towards the spatio‐temporal variability in the different oceanographic regions they encounter could indicate resilience against environmental changes.
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6.
Trine Ytrestøyl Harald Takle Jelena Kolarevic Sara Calabrese Gerrit Timmerhaus Bjørn O. Rosseland Hans C. Teien Tom O. Nilsen Sigurd O. Handeland Sigurd O. Stefansson Lars O. E. Ebbesson Bendik F. Terjesen 《Journal of the World Aquaculture Society》2020,51(2):373-392
Producing a larger post-smolt in recirculating aquaculture systems (RAS) could shorten the production time in sea cages and potentially reduce mortality. Knowledge of the biological requirements of post-smolts in closed-containment systems is however lacking. In the present study, the effects of salinity and water velocity on growth, survival, health, and welfare of Atlantic salmon reared in RAS were examined. Salmon smolts were stocked in three separate RAS with salinities of 12, 22, and 32‰ and subjected to high (1.0 body lengths per s−1) or low (0.3 body lengths second−1) water velocity. Growth performance, survival, welfare, and physiological stress responses were monitored until the fish reached a bodyweight of around 450 g. Growth rate was higher at lower salinity and higher water velocity generally had a positive effect on growth in all salinities. Feed conversion ratio was lower at 12‰ compared to the 22 and 32‰ when the fish were between 250 and 450 g. Higher mortality, elevated plasma cortisol levels, higher incidence of cataract, and a higher expression of stress-induced genes in the skin (iNOS, Muc5ac-like) indicated a negative effect of higher salinity on fish welfare. Male maturation was low (<1%), and not affected by salinity or water velocity. 相似文献
7.
Jann Th. Martinsohn Paul Raymond Trey Knott Kevin A. Glover Einar Eg Nielsen Lars Bonde Eriksen Rob Ogden John Casey Jordi Guillen 《Fish and Fisheries》2019,20(2):391-401
Evidence from DNA‐analysis is commonplace in human criminal investigations, and while it is increasingly being used in wildlife crime, to date, its application to control and enforcement activities in fisheries and aquaculture has only been sporadic. Contemporary DNA‐analysis tools are capable of addressing a broad range of compliance issues, species identification, mislabelling of fish products, determining the origin of catches and the farm of origin of aquaculture escapees. Such applications have the potential to ensure traceability along the fish product supply chain and to combat consumer fraud and Illegal, Unreported and Unregulated fishing. Nevertheless, DNA‐analysis is not yet used routinely in investigations into compliance with fisheries and aquaculture legislation. One potential reason for this is that DNA‐analysis techniques may have been regarded as too expensive. However, costs have plummeted over the past decade prompting us to objectively assess whether the costs associated with routine use of DNA‐analysis techniques for fisheries and aquaculture control and enforcement activities do constitute an impediment. Based on a number of recent fisheries and aquaculture compliance investigations that incorporated DNA‐analysis, our results indicate that the use of genetic analysis was justified and worthwhile in all cases examined. We therefore conclude that the costs associated with DNA‐analysis do not represent a barrier to the routine adoption of DNA‐analysis techniques in fisheries and aquaculture compliance investigations. Thus, control and enforcement agencies should be encouraged to use such techniques routinely. 相似文献
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